Composite

Part:BBa_J15201:Design

Designed by: Chris French   Group: French Lab   (2007-07-13)


rbs+arsR, E. coli chromosomal arsR repressor coding sequence with rbs.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note the presence of a SacI site at the 5' end of this construct, overlapping the XbaI site. This is preserved when this biobrick is added to any other biobrick, allowing easy testing for proper insertion by restriction digestion.


Source

RBS synthetic; arsR from Escherichia coli K12, sequence derived from X80057 (gi:510824).

References